PERBANDINGAN METODE ANALISIS AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SIRIH MERAH (Piper crocatum) MENGGUNAKAN METODE DPPH, ABTS, DAN FRAP SERTA KORELASINYA DENGAN KANDUNGAN FENOLIK TOTAL

Pamungkas, Diah Rahayu (2026) PERBANDINGAN METODE ANALISIS AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SIRIH MERAH (Piper crocatum) MENGGUNAKAN METODE DPPH, ABTS, DAN FRAP SERTA KORELASINYA DENGAN KANDUNGAN FENOLIK TOTAL. S1 thesis, Universitas PGRI Madiun.

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Abstract

Red betel leaf (Piper crocatum) is a botanical species that possesses bioactive elements which may serve as natural antioxidants. This research was designed to evaluate IC₅₀ values for DPPH and ABTS methods, to assess the total phenolic content, and to investigate the relationship between antioxidant capacity and total phenolic content.The investigation involved the process of ethanol extraction, followed by the assessment of antioxidant activity using DPPH, ABTS, and FRAP techniques, alongside the measurement of total phenolic content utilizing the Folin Ciocalteu approach. The information collected was examined through linear regression, One-Way ANOVA, Tukey HSD post hoc tests, and pearson correlation assessments. The findings revealed that the IC50 values for both DPPH and ABTS methods were 96.8 ppm (R² = 0.943) and 69.91 ppm (R² = 0.993), respectively, while the FRAP method showed antioxidant activity ranging from 2.77 to 16.19 mg VCE/g extract (R² = 0.975). The statistical evaluation demonstrated that variations in concentration had a significant impact on antioxidant activity (p < 0.05). The total phenolic content exhibited a very strong positive correlation with antioxidant activity, with the most substantial correlation detected in the ABTS method (r = 0.978). In summary, the ethanolic extract of red betel leaf reveals significant antioxidant potential, with the ABTS method recognized as the most sensitive and displaying the strongest correlation with total phenolic content. Keywords: antioxidant, Piper crocatum, DPPH, ABTS, total phenolic content

Item Type:	Thesis/Skripsi/Tugas Akhir (S1)
Kata Kunci:	Antioxidant; Piper crocatum; DPPH; ABTS; total phenolic content
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry
Divisions:	Fakultas Ilmu Kesehatan dan Sains > Farmasi
Depositing User:	PAMUNGKAS RAHAYU DIAH
Date Deposited:	26 Aug 2026 03:03
Last Modified:	26 Aug 2026 03:03
URI:	http://eprint.unipma.ac.id/id/eprint/8388

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