SHOLIKHAH, ANNISA KHOFIYATUS (2025) METABOLITE PROFILING BUNGA TELANG (Clitoria ternatea L.) MENGGUNAKAN UPLC-QtoF-MS/MS DAN AKTIVITAS ANTIOKSIDAN MENGGUNAKAN METODE DPPH DARI TIGA DAERAH BERBEDA DI INDONESIA. S1 thesis, Universitas PGRI Madiun.
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Abstract
Annisa Khofiyatus Sholikhah. 2025. Metabolite Profiling of Butterfly Pea Flower (Clitoria Ternatea L.) Using UPLC-QTOF-MS/MS and Antioxidant Activity Using DPPH Method From Three Different Regions in Indonesia. Thesis. Pharmacy Study Program., Faculty of Health and Science, Universitas PGRI Madiun. Supervisor (I) Dr. drh. Cicilia Novi Primiani, M.pd., Supervisor (II) Apt. Weka Shida Bhagawan, M. Farm. This study aims to examine the profile of secondary metabolite compounds and antioxidant activity of butterfly pea flower extract (Clitoria ternatea L.) originating from three different regions in Indonesia: East Java, Central Java, and West Java. Extraction was carried out using the remaceration method with 96% ethanol solvent, while compound analysis was carried out using a UPLC-QToF-MS/MS instrument. Antioxidant activity was evaluated using the DPPH method and measured using a UV-Vis spectrophotometer, with the IC₅₀ parameter as an indicator of antioxidant strength. The results of the study showed that there were variations in the composition of secondary metabolite compounds in each region. In East Java, the main compound identified was Mitragynine (18.56%), while in Central Java it was dominated by Ethyl-(2S)-2-[(3S,5aS,8aS,9aS)-3-methyl-1,4-dioxodecahydro-2H-cyclopentapyrrolo[1,2-a]pyrazin-2-yl]-4-phenylbutanoat (52.30%). In West Java, the major compound was N-Acetyl-L-histidyl-L-tryptophyl-L-alanyl-L-valylglycyl-N-[(4R)-2-methyl-4-decanyl]-L-histidinamide (16.38%). In addition, marker compounds such as Tris(4-nitrophenyl)amine, Phenylglyoxylic acid, Quercetin, and Euquinene were also found in East Java and Central Java, but were not detected in West Java. Antioxidant activity test showed that butterfly pea flower extract from the three regions had moderate activity category with IC₅₀ values respectively: East Java 131.54 µg/mL, Central Java 129.98 µg/mL, and West Java 127.55 µg/mL. There was no significant difference between the antioxidant activity of butterfly pea flower extract from the three regions or compared with vitamin C as a control. These results indicate that butterfly pea flower extract from the three regions can potentially be a source of natural antioxidants that are as effective as vitamin C. This study confirms the importance of geographical factors in influencing the secondary metabolite profile of plants and supports the development of butterfly pea flowers as raw materials for functional foods and antioxidant-based pharmaceuticals. Keywords: Metabolite Profiling, Butterfly Pea Flower (Clitoria ternatea), UV-Vis Spectrophotometer, UPLC-QtoF-MS/MS
| Item Type: | Thesis/Skripsi/Tugas Akhir (S1) |
|---|---|
| Kata Kunci: | Metabolite Profiling; Butterfly Pea Flower (Clitoria ternatea); UV-Vis Spectrophotometer; UPLC-QtoF-MS/MS |
| Subjects: | L Education > L Education (General) Q Science > Q Science (General) |
| Divisions: | Fakultas Ilmu Kesehatan dan Sains > Farmasi |
| Depositing User: | SHOLIKHAH KHOFIYATUS ANNISA |
| Date Deposited: | 29 Aug 2025 04:23 |
| Last Modified: | 29 Aug 2025 04:23 |
| URI: | http://eprint.unipma.ac.id/id/eprint/4553 |
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